An ultrasensitive fluorescence aptasensor for SARS-CoV-2 antigen based on hyperbranched rolling circle amplificationArticle Published on 2023-04-012023-07-11 Journal: Talanta [Category] COVID19(2023년), [키워드] aptasensor HRCA pseudovirus S1-protein SARS-CoV-2. [DOI] 10.1016/j.talanta.2022.124221 PMC 바로가기
SARS-CoV-2 pseudovirus enters the host cells through spike protein-CD147 in an Arf6-dependent mannerSARS-CoV-2 슈도바이러스는 Arf6 의존적 방식으로 스파이크 단백질-CD147을 통해 숙주 세포에 들어갑니다.Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 approach Arf6 blockade CD147 cells clathrin Clathrin-mediated endocytosis clinically coronavirus endocytic endocytic mechanism endocytosis evaluate feasible function host cell host cells Huh-7 cell Huh-7 cells independent independent of indicated infected Vero E6 cells Infection inhibited inhibitor knockdown Localization macropinocytosis pathway Prevent pseudovirus pseudovirus infection public health RAB5A SARS-CoV-2 SARS-CoV-2 entry SARS-COV-2 infection SARS-CoV-2 pseudovirus SARS-CoV-2 pseudovirus infection severe acute respiratory syndrome Coronavirus Spike protein spike protein. Spread the SARS-CoV-2 variant Vero E6 VERO E6 cells viral infection weakened [DOI] 10.1080/22221751.2022.2059403 PMC 바로가기 [Article Type] Article
SARS-CoV-2 fusion-inhibitory lipopeptides maintain high potency against divergent variants of concern including OmicronSARS-CoV-2 융합 억제 리포펩티드는 오미크론을 포함한 다양한 관심 변종에 대해 높은 효능을 유지합니다Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] activity Alpha antiviral activity Beta broad-spectrum antiviral broad-spectrum antivirals Cell Cell fusion cell-cell fusion combating COVID-19 pandemic D614G Delta drugs effective vaccine Effective vaccines exhibited FIVE fusion inhibitor Gamma Impact Infection inhibiting inhibitor inhibitors Lambda lipopeptide maintain mutant omicron Omicron variant other variant other variants outbreak pseudovirus pseudovirus pseudovirus infection pseudoviruses reduced S protein S proteins SARS-CoV-2 significantly the S protein therapeutic variant variants variants of concern variants of concern. VoC VOCs wild-type WT virus [DOI] 10.1080/22221751.2022.2098060 PMC 바로가기 [Article Type] Article
Evaluation of commercially available fully automated and ELISA-based assays for detecting anti-SARS-CoV-2 neutralizing antibodiesArticle Published on 2022-11-082022-11-15 Journal: Scientific Reports [Category] SARS, 치료기술, [키워드] acute respiratory syndrome administration affecting analysis Analysis anti-RBD IgG antibody anti-SARS-CoV-2 AUC automated binding antibody booster clinical settings Cohort collected Control coronavirus correlation COVID-19 cutoff value demonstrated Effectiveness Genscript gold standard Immunity immunoassay in both groups infected patient Mindray NAb neutralization Neutralization assay neutralization test neutralizing antibody population level protective immunity provide pseudovirus R 2 Rapid receiver operating characteristic Research ROC ROC Curve Sample SARS-CoV-2 SARS-CoV-2 infected patient second dose sensitivity shown significantly specificity subjects sVNT sVNT assay symptoms onset tested threshold vaccinated individual Vaccine virus neutralization test was performed [DOI] 10.1038/s41598-022-21317-x PMC 바로가기
Potential of antibody pair targeting conserved antigenic sites in diagnosis of SARS-CoV-2 variants infectionArticle Published on 2022-11-012022-11-15 Journal: Journal of virological methods [Category] SARS, 변종, 진단, 치료기술, 치료제, [키워드] antibody Antibody pair Antigen antigenic site antigenic sites binding domain candidate caused clinical samples Clusters complex conformational conserved Coronavirus disease 2019 COVID-19 COVID-19 pandemic demonstrated determine develop diagnosis of SARS-CoV-2 diagnostic early diagnosis ELISA Infection mAb mAbs monoclonal antibody pandemic Potential pseudovirus RBD RBD-specific mAbs recognizing residue SARS-CoV SARS-CoV S protein SARS-CoV-2 SARS-CoV-2 receptor SARS-CoV-2 S protein SARS-CoV-2 variant selected Sensitivity and specificity spike Spike antigen. Spike protein substitutions Treatment variant VOCs [DOI] 10.1016/j.jviromet.2022.114597 PMC 바로가기
Antibody correlates of protection from SARS-CoV-2 reinfection prior to vaccination: A nested case-control within the SIREN studyArticle Published on 2022-11-012022-11-15 Journal: The Journal of Infection [Category] SARS, 진단, 치료기술, [키워드] age analysed anti-S anti-SARS-CoV-2 antibody association Case-control case-control study controls correlated fixed Gender identify Immunity Increasing infected individual Infection Linear regression Live virus logistic regression models Neutralising Antibodies no difference nucleocapsid occurred performed pre-vaccination protective effect pseudovirus reduced risk Reinfection required Result SARS-CoV-2 SARS-CoV-2 serology. serological serology serum sample significantly lower SIREN study tested titre titres vaccination Wuhan [DOI] 10.1016/j.jinf.2022.09.004 PMC 바로가기
Hamsters Protected from SARS-CoV-2 Delta Variant Challenge after Two Doses of Adjuvanted SARS-CoV-2 Recombinant Spike Protein (S-2P) and One Dose of Beta S-2PArticle Published on 2022-11-012022-11-15 Journal: The Journal of Infectious Diseases [Category] SARS, 변종, 진단, [키워드] acute respiratory syndrome Beta challenge coronavirus COVID-19 COVID-19 vaccine Delta dose Effectiveness hamster hamster challenge study highest live SARS-CoV-2 Live virus lung MVC-COV1901 neutralization Neutralizing antibody titer Omicron variant pathology prefusion PROTECT pseudovirus recombinant regimen S-2P SARS-CoV-2 SARS-CoV-2 delta SARS-CoV-2 vaccine spike Spike protein subunit Subunit vaccine suggested vaccination Vaccines variant Variant of concern. variants variants of concern virus Wuhan [DOI] 10.1093/infdis/jiac153 PMC 바로가기
Transcriptional regulation and small compound targeting of ACE2 in lung epithelial cellsArticle Published on 2022-11-012022-11-15 Journal: Acta pharmacologica Sinica [Category] COVID19(2023년), SARS, 진단, [키워드] 16HBE 16HBE cell 16HBE cells 6-O-angeloylplenolin ACE2 ACE2 expression addition administration Cell Centipeda Centipeda minima chemokine Chinese Compound conducted COVID-19 CX3CL1 CXCL13 dataset demonstrated downregulated entry of SARS-CoV-2 enzyme evaluated expression gene encoding HLF IL-6 inhibited inhibitor Interaction lung epithelial cell Lung epithelial cells lung tissue lung tissues mice modulate parental pathogen phosphorylated positively correlated promoter PROTECT Protein pseudovirus receptor reduced regulate Regulation S protein SARS-CoV-2 SARS-COV-2 infection screened STAT3 STAT3. stimulated suppressed target cells tested TFS Transcription transcription factor transfected with [DOI] 10.1038/s41401-022-00906-6 PMC 바로가기
Genomic Epidemiology and Serology Associated with a SARS-CoV-2 R.1 Variant Outbreak in New JerseyArticle Published on 2022-10-262022-11-15 Journal: mBio [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 진단, 치료기술, [키워드] acute respiratory syndrome antibody antibody resistance attack rate B.1.351 B.1.526 Care caused characteristic Clinical use collected Community Complete coronavirus COVID-19 Critical E484K mutation Evidence Examining exhibit exhibited hcp Health highlight individual infected individuals Infection Inpatient Lineage loss of activity monoclonal antibody Mutation N-terminal domain neutralization capacity Neutralizing antibody response neutralizing capacity New nosocomial NTD outbreak P.1 Patient plasma positive professional professionals proportion pseudovirus psychiatric facility Receptor binding domain reported reverse transcription robust SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant sera Spike protein Symptom symptomatic COVID-19 disease tested the SARS-CoV-2 the United State treat vaccinated individual vaccinated individuals vaccination Vaccine variant variants of concern variants of concern. VOCs wild type [DOI] 10.1128/mbio.02141-22 PMC 바로가기
A pseudovirus-based platform to measure neutralizing antibodies in Mexico using SARS-CoV-2 as proof-of-conceptArticle Published on 2022-10-262022-11-15 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 진단, 치료제, [키워드] Accuracy antiviral immune response applied BSL-2 BSL-3 BSL-3 facilities evaluate evidenced functional in vitro Infection information Latin lentiviral particles Live virus Luminescence methodology NAb NAbs neutralizing antibody non-replicative Particle pathogen performed platform Protein pseudotyped lentiviral particles pseudovirus reporter resource Safe SARS-CoV-2 selected sensitivity serum samples specificity target cell transduced viral pathogen [DOI] 10.1038/s41598-022-22921-7 PMC 바로가기